Brenda J. Sweeney, MS, SCT(ASCP)MBCM
Massachusetts General Hospital
Flow cytometry is complex and can be overwhelming to the novice. This book is nicely laid out with the intent of walking the reader through these complex processes and allows you to form a foundation of the basics and builds from there.
The book contains 10 chapters with 116 figures to help those starting out in the field grasp the basic concepts involved in performing flow cytometry.
It begins with definitions, history and types of cytometers. Chapter 2 goes on to explain the mechanics of the analyzer and how the system captures data to help the reader understand the instrument and the principles of the technology. First explaining fluidics, optics and electronics, and then finishing with a section on bringing all the information together. The next chapter covers the terminology used throughout the book, so the reader understands the “language” of flow cytometry. Included are explanations of quality checks and the importance of laser alignment along with basic panel design, acquisition & analysis plus the basic concepts of compensation. Chapter 4 is dedicated to the pros/cons of fluorochrome choices and how to combine or avoid using together. I especially liked chapter 6, which covers antibody development for flow cytometry. It’s a rich chapter containing 19 figures to help illustrate the concepts.
From there, experimental considerations are reviewed, covering receptor blocking and the importance of excluding dead cells in the interpretation of the data. Further explanation is given to cell number, antibody concentration and background staining. Cell enrichment, surface staining and intracellular staining are covered within the next few chapters. The final chapter, devoted to troubleshooting, is nicely done using a series of questions, each followed by a detailed explanation and a great set of diagrams to explain each concept.
Overall, the book is full of useful information and explanations. I would expect in the face of the changes on the horizon in cytology it would be useful to an advanced level cytology specialist to gain a greater understanding of the ancillary test so commonly ordered on pathology specimens.
Publisher: Springer Nature Switzerland AG, 2018
211 pages, 113 color illustrations
ISBN 978-3-319-98071-3 (eBook)